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MicroSieves
分離、分類、收集、濃縮 (精密的矩陣排列濾網)
Autoclavable and can be repeatedly washed and
reused.
scanning electron micrograph of
MicroSieves
MicroSieves
allows the rapid, yet gentle, isolation of
cells, organelles, or cellular debris. Unlike traditional filters,
MicroSieves is a precision woven matrix
of pure nylon filaments that is available with pores ranging from 5
to 200 microns. MicroSieves does not wet and contains no dyes,
detergents or extractables. Extremely low protein affinity means that
cells and proteins will not bind to MicroSieves. They have a wide
variety of distinctive uses a specific sized porous screens in the
laboratory:
-
Rapid
collection
of tissue culture cells without
centrifugation.
-
Isolation of
specific cell types from a dissociated tissue sample, allowing only
a single type of cell to pass through.
-
Separation
of different sized cells
from a mixture of cell types.
-
Concentration
and isolation of cells from a
bioreactor.
-
Barrier
to separate cells or
tissues, while allowing free media diffusion.
-
Prefilter
to prevent clogging of final
filter.
MicroSieves
are versatile tools in the laboratory. A pure nylon product that is
biologically inert.
Autoclavable and can be repeatedly washed and
reused. MicroSieves are resistant to most acidic or basic
conditions and many solvents.
|
Cell Sorting using MicroSieves
|
|
To isolate
These cells |
Size
(approximate) |
MicroSieves
used |
|
Thymocytes
(mouse) |
6μ |
N5 |
|
Chloroplasts
(spinach) |
8μ |
N5 |
|
Ehrlich Ascites
(mouse) |
16μ |
N10 |
|
Hybridoma
(mouse) |
<20μ |
N8 |
|
Liver Cells |
20μ |
N15 |
|
Oocytes
(mouse) |
75μ |
N50 |
|
Adipocytes
(human) |
94μ |
N70 |
ORDERING
INFORMATION
|
Micron Pore
Size |
Three 12 x 12 inch square
sheets (30 X 30
cm, 3張/包) |
Roll 2 x
3 Feet (61 X 91.5
cm) |
Ten 47mm Circles
(not sterile)
47mm 圓形, 10個/包 |
|
CATALOG |
CATALOG |
CATALOG |
|
200 |
N200S |
|
N200R |
|
N200C |
|
|
100 |
N100S |
|
N100R |
|
N100C |
|
|
70 |
N70S |
|
N70R |
|
N70C |
|
|
50 |
N50S |
|
N50R |
|
N50C |
|
|
35 |
N35S |
|
N35R |
|
N35C |
|
|
25 |
N25S |
|
N25R |
|
N25C |
|
|
20 |
N20S |
|
N20R |
|
N20C |
|
|
15 |
N15S |
|
N15R |
|
N15C |
|
|
10 |
N10S |
|
N10R |
|
N10C |
|
|
8 |
N8S |
|
N8R |
|
N8C |
|
|
5 |
N5S |
|
N5R |
|
N5C |
|
|
|
SAMPLER PACKS of MicroSieves
contains one 12 inch x 12 inch square
sheet of each pore size listed: (30 x30
cm, 5 種 micron 各1張)
|
CATALOG # |
MICRON PORE SIZES |
|
NSA |
200,
100, 50, 25, 8 micron |
|
|
NSB |
100,
70, 50, 35, 25 micron |
|
|
NSC |
50, 35,
20, 15, 8 micron |
|
|
NSD |
20, 15,
10, 8, 5 micron |
|
|
|
SIEVE HOLDERS
:
Polypropylene 2 piece frame with
adjustable metal tightener. Securely holds the MicroSieves taut or
used to form a funnel. Autoclavable.
Sieve holder being used with
MicroSieves
|
CATALOG # |
DIAMETER |
|
F3 |
3 inch (7.6 cm) |
|
|
F4 |
4 inch (10 cm) |
|
|
F6 |
6 inch (15.2 cm) |
|
|
F8 |
8 inch (20.3 cm) |
|
|
F10 |
10 inch (25.4 cm) |
|
|
F101 |
all 5 sizes |
|
|
|
FILTRATION UNITS:
Type
A (on left) and Type B (on right)
Type A:
Borosilicate glass filtration apparatus with stainless steel filter
support. An aluminum clamp sandwiches a MicroSieves 47mm circle
between the 300 ml top funnel and base. Autoclavable.
Type B:
Polysulfone non-toxic plastic filtration unit that can be repeatedly
re-used and autoclaved. Uses a 47mm MicroSieves circle. Funnel
and base both have a 300 ml capacity.
|
FILTRATION UNITS |
TYPE A FILTER UNIT |
TYPE B FILTER UNIT |
|
QUANTITY: |
CATALOG # |
CATALOG # |
|
One |
F103 |
|
F107 |
|
|
Three |
F105 |
|
F109 |
|
|
|
|
BioNylon
(精密的矩陣排列濾網)
Autoclavable and can be repeatedly washed and
reused.
A SYNTHETIC
CHEESECLOTH
Many biological preparations begin with extractions that require
coarse filtration to remove cellular debris. But bulk filtration using
cheesecloth absorbs lots of the extraction fluid, while membranes
quickly clog.
BioNylon
produces rapid bulk filtration at extraordinary flow rates.
BioNylon is loosely placed in a sieve holder to form a funnel. The
sample is placed in the funnel, allowed to drain, and the layers of
the BioNylon are gently pressed together to collect all of the
extraction liquid.
-
Consists of very fine
nylon filaments woven into a matrix with 50 micron pores.
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A pure nylon product
without detergents, dyes, or salts. Can be used directly with
biologicals.
-
As BioNylon does not
wet, significantly greater quantities of the extraction liquid are
recovered compared to any other method.
-
BioNylon does not
bind proteins, so no loss of your valuable sample will occur.
-
Compatible with the
salts, buffers, and solvents used in biological research.
Autoclavable.
-
Extraordinary cost
effective compared to other bulk filtration methods. High tensile
strength. Can be repeatedly washed and reused.
BioNylon ORDERING INFORMATION
| BioNylon |
CATALOG |
|
THREE 12 x
12 inch square SHEETS (30 X
30
cm) |
N301 |
|
|
SIX 12 x 12
inch square SHEETS (30 X
30
cm) |
N303 |
|
|
ROLL 2 x 3
FEET(61 X 91.5
cm) |
N305 |
|
|
ROLL 4 x 3
FEET(122 X 91.5
cm) |
N307 |
|
|
|
SIEVE HOLDERS
|
DIAMETER |
CATALOG |
|
3 INCH
(7.6 cm) |
F3 |
|
|
4 INCH
(10.0 cm) |
F4 |
|
|
6 INCH
(15.2 cm) |
F6 |
|
|
8 INCH
(20.3 cm) |
F8 |
|
|
10 INCH
(25.4 cm) |
F10 |
|
|
SET OF ALL
5 SIZES |
F101 |
|

BioNylon used in the
Sieve Holder |
透析膜 |